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CAPN2 Monoclonal antibody

CAPN2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E1F10

Cat no : 66977-1-Ig

Synonyms

Calpain 2 catalytic subunit, Calpain 2 large subunit, Calpain large polypeptide L2, Calpain M type, CANP 2, CANP2, CANPL2, CANPml, CAPN2, FLJ39928, M calpain, mCANP, Millimolar calpain

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CAPN2 Monoclonal antibody (66977-1-Ig)

    WB analysis using 66977-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using CAPN2 Monoclonal antibody (66977-1-Ig)

    WB analysis using 66977-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using CAPN2 Monoclonal antibody (66977-1-Ig)

    WB analysis of HSC-T6 using 66977-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using CAPN2 Monoclonal antibody (66977-1-Ig)

    WB analysis of NIH/3T3 using 66977-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using CAPN2 Monoclonal antibody (66977-1-Ig)

    WB analysis of human placenta using 66977-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CAPN2 Monoclonal antibody (66977-1-Ig)

    IHC staining of human pancreas cancer using 66977-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CAPN2 Monoclonal antibody (66977-1-Ig)

    IHC staining of human colon cancer using 66977-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CAPN2 Monoclonal antibody (66977-1-Ig)

    IHC staining of human stomach cancer using 66977-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using CAPN2 Monoclonal antibody (66977-1-Ig)

    IHC staining of rat colon using 66977-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CAPN2 Monoclonal antibody (66977-1-Ig)

    IF Staining of HepG2 using 66977-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CAPN2 antibody (66977-1-Ig, Clone: 1E1F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HSC-T6 cells, NIH/3T3 cells, human placenta tissue, U2OS cells, LNCaP cells, HEK-293 cells, rat brain tissue, mouse brain tissue, HepG2 cells
Positive IHC detected inhuman pancreas cancer tissue, human stomach cancer tissue, human colon cancer tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66977-1-Ig targets CAPN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CAPN2 fusion protein Ag28427
Full Name calpain 2, (m/II) large subunit
Calculated Molecular Weight 700 aa, 80 kDa
Observed Molecular Weight 72-80 kDa
GenBank Accession NumberBC021303
Gene Symbol CAPN2
Gene ID (NCBI) 824
RRIDAB_2882297
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calpain 2(Calpain-2 catalytic subunit) is also named as CANPL2, CANPml, mCANP, FLJ39928, and belongs to the peptidase C2 family. N-terminal sequencing of CAPN2 purified from the human liver indicates that the N-terminal methionine is removed, resulting in a mature 699-amino acid subunit with a calculated molecular mass of 79.9 kD(PMID:2852952). It is a calcium-regulated non-lysosomal thiol-protease that catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has 2 isoforms produced by alternative splicing with the molecular weight of 80 kDa and 71 kDa.

Protocols

Product Specific Protocols
WB protocol for CAPN2 antibody 66977-1-IgDownload protocol
IHC protocol for CAPN2 antibody 66977-1-IgDownload protocol
IF protocol for CAPN2 antibody 66977-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Oxid Med Cell Longev

Polysaccharides from Polygonatum cyrtonema Hua Reduce Depression-Like Behavior in Mice by Inhibiting Oxidative Stress-Calpain-1-NLRP3 Signaling Axis.

Authors - Fengming Shen
View Article
humanIF

Cell Mol Life Sci

Calpains as novel players in the molecular pathogenesis of spinocerebellar ataxia type 17.

Authors - Jonasz Jeremiasz Weber
View Article