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  • Featured Product
  • KD/KO Validated

STIL Monoclonal antibody

STIL Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E12C1

Cat no : 66876-1-Ig

Synonyms

TAL-1-interrupting locus protein, SIL, SCL-interrupting locus protein, 1E12C1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STIL Monoclonal antibody (66876-1-Ig)

    WB analysis using 66876-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66876-1-Ig (STIL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using STIL Monoclonal antibody (66876-1-Ig)

    IHC staining of human colon cancer using 66876-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66876-1-Ig (STIL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using STIL Monoclonal antibody (66876-1-Ig)

    IHC staining of human colon cancer using 66876-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66876-1-Ig (STIL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STIL Monoclonal antibody (66876-1-Ig)

    IHC staining of human lung cancer using 66876-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66876-1-Ig (STIL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STIL Monoclonal antibody (66876-1-Ig)

    IHC staining of human lung cancer using 66876-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66876-1-Ig (STIL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using STIL Monoclonal antibody (66876-1-Ig)

    IF Staining of Jurkat using 66876-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using STIL antibody (66876-1-Ig, Clone: 1E12C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using STIL Monoclonal antibody (66876-1-Ig)

    IF Staining of A431 using 66876-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using STIL antibody (66876-1-Ig, Clone: 1E12C1 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, HL-60 cells, Daudi cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66876-1-Ig targets STIL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STIL fusion protein Ag19124
Full Name SCL/TAL1 interrupting locus
Calculated Molecular Weight 1287 aa, 143 kDa
Observed Molecular Weight 150-155 kDa
GenBank Accession NumberBC126223
Gene Symbol STIL
Gene ID (NCBI) 6491
RRIDAB_2882210
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for STIL antibody 66876-1-IgDownload protocol
IHC protocol for STIL antibody 66876-1-IgDownload protocol
IF protocol for STIL antibody 66876-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cancer Cell Int

ncRNAs-mediated overexpression of STIL predict unfavorable prognosis and correlated with the efficacy of immunotherapy of hepatocellular carcinoma

Authors - Longwen Xu
View Article
humanWB

Cancer Cell Int

The biological function and clinical significance of STIL in osteosarcoma.

Authors - Shu-Fan Ji
  • KD Validated
View Article
humanWB

J Cell Physiol

Primary cilium participates in radiation-induced bystander effects through TGF-β1 signaling

Authors - Pei Qu
  • KD Validated
View Article
humanIF

Cell Rep

Dual roles of CCDC102A in governing centrosome duplication and cohesion

Authors - Tianning Wang
View Article