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  • Featured Product
  • KD/KO Validated

CCNY Monoclonal antibody

CCNY Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2C9E3

Cat no : 66865-1-Ig

Synonyms

C10orf9, CBCP1, CCNY, CFP1, Cyc Y, Cyclin box protein 1, Cyclin fold protein 1, cyclin X, cyclin Y, FLJ95513

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCNY Monoclonal antibody (66865-1-Ig)

    WB analysis using 66865-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66865-1-Ig (CCNY antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using CCNY Monoclonal antibody (66865-1-Ig)

    WB analysis of A549 using 66865-1-Ig

    WB result of CCNY antibody (66865-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCNY transfected A549 cells.

  • Western Blot (WB) analysis of NIH/3T3 cells using CCNY Monoclonal antibody (66865-1-Ig)

    WB analysis of NIH/3T3 using 66865-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66865-1-Ig (CCNY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using CCNY Monoclonal antibody (66865-1-Ig)

    WB analysis of RAW 264.7 using 66865-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66865-1-Ig (CCNY antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, RAW 264.7 cells, A549 cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66865-1-Ig targets CCNY in WB, IF, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CCNY fusion protein Ag12691
Full Name cyclin Y
Calculated Molecular Weight 33 kDa, 39 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC104773
Gene Symbol CCNY
Gene ID (NCBI) 219771
RRIDAB_2882202
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCNY, also named as C10orf9, CBCP1, CFP1, is a novel cyclin. CCNY acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma membrane and promoting phosphorylation of LRP6, leading to the activation of the Wnt signaling pathway.

Protocols

Product Specific Protocols
WB protocol for CCNY antibody 66865-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Nat Commun

AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy.

Authors - Marc Dohmen
View Article