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Tested Applications
| Positive WB detected in | HeLa cells, NIH/3T3 cells, RAW 264.7 cells, A549 cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
66865-1-Ig targets CCNY in WB, IF, ELISA applications and shows reactivity with Human, Rat, Mouse samples.
| Tested Reactivity | Human, Rat, Mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CCNY fusion protein Ag12691 Predict reactive species |
| Full Name | cyclin Y |
| Calculated Molecular Weight | 33 kDa, 39 kDa |
| Observed Molecular Weight | 37 kDa |
| GenBank Accession Number | BC104773 |
| Gene Symbol | CCNY |
| Gene ID (NCBI) | 219771 |
| RRID | AB_2882202 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8ND76 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CCNY, also named as C10orf9, CBCP1, CFP1, is a novel cyclin. CCNY acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma membrane and promoting phosphorylation of LRP6, leading to the activation of the Wnt signaling pathway.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CCNY antibody 66865-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
