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  • Western Blot (WB) analysis of various lysates using JTV1 Monoclonal antibody (66848-1-Ig)

    WB analysis using 66848-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66848-1-Ig (JTV1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using JTV1 Monoclonal antibody (66848-1-Ig)

    WB analysis using 66848-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66848-1-Ig (JTV1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using JTV1 Monoclonal antibody (66848-1-Ig)

    IHC staining of mouse testis using 66848-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66848-1-Ig (JTV1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using JTV1 Monoclonal antibody (66848-1-Ig)

    IHC staining of mouse testis using 66848-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66848-1-Ig (JTV1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HepG2 cells using JTV1 Monoclonal antibody (66848-1-Ig)

    FC experiment of HepG2 using 66848-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug JTV1 Monoclonal antibody (66848-1-Ig, Clone:1D6B5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

JTV1 Monoclonal antibody

JTV1 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 66848-1-Ig
Clone No.1D6B5

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

PRO0992, P38, HLD17, Aminoacyl tRNA synthase complex-interacting multifunctional protein 2, AIMP2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, HEK-293 cells, HepG2 cells, HL-60 cells. HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66848-1-Ig targets JTV1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen JTV1 fusion protein Ag0675 Predict reactive species
Full Name JTV1 gene
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 32-36 kDa
GenBank Accession NumberBC002853
Gene Symbol JTV1
Gene ID (NCBI) 7965
RRIDAB_2882188
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13155
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

JTV1, also named p38, AIMP2, was first knew as a factor associated with macromolecular protein complex, which consists several different aminoacyl-tRNA synthetases. JTV1 is a scaffold protein required for the assembly and stability of the multi-tRNA synthetase complex. JTV1 could act as a mediator of TGF-beta signaling and its functional importance in the control of MYC during lung differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor.

Protocols

Product Specific Protocols
WB protocol for JTV1 antibody 66848-1-IgDownload protocol
IHC protocol for JTV1 antibody 66848-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols