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B7‑H4 Monoclonal antibody

B7‑H4 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E5B1

Cat no : 66817-1-Ig

Synonyms

VTCN1, B7-H4, B7H4, B7h.5, B7 homolog 4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using B7‑H4 Monoclonal antibody (66817-1-Ig)

    WB analysis using 66817-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66817-1-Ig (B7‑H4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using B7‑H4 Monoclonal antibody (66817-1-Ig)

    IHC staining of human cervical cancer using 66817-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66817-1-Ig (B7‑H4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using B7‑H4 Monoclonal antibody (66817-1-Ig)

    IHC staining of mouse colon using 66817-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66817-1-Ig (B7‑H4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using B7‑H4 Monoclonal antibody (66817-1-Ig)

    IHC staining of rat kidney using 66817-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66817-1-Ig (B7‑H4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inSKOV-3 cells, T-47D cells, HeLa cells, A549 cells
Positive IHC detected inrat kidney tissue, human cervical cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

66817-1-Ig targets B7‑H4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen B7‑H4 fusion protein Ag27751
Full Name V-set domain containing T cell activation inhibitor 1
Calculated Molecular Weight 282 aa, 31 kDa
Observed Molecular Weight 31-35 kDa
GenBank Accession NumberBC065717
Gene Symbol B7-H4
Gene ID (NCBI) 79679
RRIDAB_2882160
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B7‑H4 also named VTCN1, B7X, or B7S1 is a 282 amino acid protein, which contains 2 immunoglobulin-like domains and belongs to the immunoglobulin superfamily. B7‑H4 negatively regulates T-cell mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. B7‑H4 is a single-pass type I membrane protein, which is over-expressed in breast, ovarian, endometrial, renal cell and non-small-cell lung cancers. The predicted molecular weight of B7‑H4 is 31 kDa. The glycosylated B7‑H4 is 50 to 80 kDa, and the non-glycosylated form is 28 kDa.

Protocols

Product Specific Protocols
WB protocol for B7‑H4 antibody 66817-1-IgDownload protocol
IHC protocol for B7‑H4 antibody 66817-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Cancer Lett

The deubiquitinase USP2a promotes tumor immunosuppression by stabilizing immune checkpoint B7-H4 in lung adenocarcinoma harboring EGFR-activating mutants

Authors - Youwei Lu
View Article