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CNKSR1 Monoclonal antibody

CNKSR1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E11H8

Cat no : 66796-1-Ig

Synonyms

CNK, CNK homolog protein 1, CNK1, CNKSR1, Connector enhancer of KSR 1, Connector enhancer of KSR like, hCNK1, KSR

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using CNKSR1 Monoclonal antibody (66796-1-Ig)

    WB analysis of MCF-7 using 66796-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66796-1-Ig (CNKSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CNKSR1 Monoclonal antibody (66796-1-Ig)

    WB analysis of HEK-293 using 66796-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66796-1-Ig (CNKSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-29 cells using CNKSR1 Monoclonal antibody (66796-1-Ig)

    WB analysis of HT-29 using 66796-1-Ig

    HT-29 cells were subjected to SDS PAGE followed by western blot with 66796-1-Ig (CNKSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Caco-2 cells using CNKSR1 Monoclonal antibody (66796-1-Ig)

    WB analysis of Caco-2 using 66796-1-Ig

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 66796-1-Ig (CNKSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells, HT-29 cells, Caco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66796-1-Ig targets CNKSR1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CNKSR1 fusion protein Ag27855
Full Name connector enhancer of kinase suppressor of Ras 1
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC011604
Gene Symbol CNKSR1
Gene ID (NCBI) 10256
RRIDAB_2882140
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CNKSR1 is a protein containing several motifs involved in protein-protein interaction, including PDZ, PH (Pleckstrin homology), and SAM (sterile alpha motif) domains. The encoded protein acts as a scaffold component for receptor tyrosine kinase signaling and may mediate crosstalk between different signaling pathways. CNKSR1 is required in multiple receptor tyrosine kinase pathways where it appears to be a tyrosine phosphorylation targe.

Protocols

Product Specific Protocols
WB protocol for CNKSR1 antibody 66796-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols