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SLC25A1 Monoclonal antibody

SLC25A1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1F11A3

Cat no : 66771-1-Ig

Synonyms

Citrate transport protein, CTP, SLC20A3, SLC25A1, Tricarboxylate carrier protein

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SLC25A1 Monoclonal antibody (66771-1-Ig)

    WB analysis using 66771-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66771-1-Ig (SLC25A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of various lysates using SLC25A1 Monoclonal antibody (66771-1-Ig)

    WB analysis using 66771-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66771-1-Ig (SLC25A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, NK-92 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66771-1-Ig targets SLC25A1 in WB, IP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen SLC25A1 fusion protein Ag7520
Full Name solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC004980
Gene Symbol SLC25A1
Gene ID (NCBI) 6576
RRIDAB_2882117
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC25A1, also known as CIC or CTP, is a mitochondrial citrate transporter that exports citrate from the mitochondria to the cytosol. SLC25A1 is highly expressed in several tumor types. Recently it has been identified as a novel transcriptional target of mutant p53 and a negative tumor prognostic marker. In addition, defects in SLC25A1 has been found as a cause of combined D-2- and L-2-hydroxyglutaric aciduria. This antibody specifically recognized the endogenous SLC25A1; no protein was detected in cells from subjects containing two alleles with truncating mutations with this antibody. (24681808, 23561848)

Protocols

Product Specific Protocols
WB protocol for SLC25A1 antibody 66771-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

Nat Commun

IL-1R-IRAKM-Slc25a1 signaling axis reprograms lipogenesis in adipocytes to promote diet-induced obesity in mice.

Authors - Weiwei Liu
View Article