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  • KD/KO Validated

BACH1 Monoclonal antibody

BACH1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, mouse, pig, rabbit and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D12A4

Cat no : 66762-1-Ig

Synonyms

BACH 1, BACH1, BTB and CNC homolog 1, HA2303



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HSC-T6 cells, Jurkat cells, K-562 cells, Raji cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66762-1-Ig targets BACH1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Rat, mouse, pig, rabbit samples.

Tested Reactivity Human, Rat, mouse, pig, rabbit
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BACH1 fusion protein Ag6011
Full Name BTB and CNC homology 1, basic leucine zipper transcription factor 1
Calculated Molecular Weight 82 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC063307
Gene Symbol BACH1
Gene ID (NCBI) 571
RRIDAB_2882108
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BTB and CNC homology 1, basic leucine zipper transcription factor 1(BACH1) is a heme-regulated transcriptional regulator that acts as a repressor or activator and a member of the AP-a superfamily. MafK belongs to the small Maf family that can function as transcriptional activators or repressors depending on the proteins with which they form heterodimers. BACH1 has crucial roles in both stress responses and transformation by H-RasV12. The molecular weight of expected mobility Bach1 is 100-110 kDa.

Protocols

Product Specific Protocols
WB protocol for BACH1 antibody 66762-1-IgDownload protocol
IHC protocol for BACH1 antibody 66762-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB,IF

Cell Rep

Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism

Authors - Hao Qin
  • KD Validated
humanWB

Life Sci Alliance

A new ferrocene derivative blocks K-Ras localization and function by oxidative modification at His95

Authors - Kristen M Rehl
humanWB

Cancer Sci

BACH1 promotes clear cell renal cell carcinoma progression by upregulating oxidative stress-related tumorigenicity

Authors - Kenshiro Takemoto
  • KD Validated