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TRAIL Monoclonal antibody, PBS Only (Capture)

TRAIL Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B9B4

Cat no : 66756-1-PBS

Synonyms

TNFSF10, TNFSF 10, TNF-related apoptosis-inducing ligand, Protein TRAIL, CD253

Validation Data Gallery

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  • Western Blot (WB) analysis of human milk using TRAIL Monoclonal antibody (66756-1-Ig)

    WB analysis of human milk using 66756-1-Ig (same clone as 66756-1-PBS)

    human milk were subjected to SDS PAGE followed by western blot with 66756-1-Ig (TRAIL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66756-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TRAIL Monoclonal antibody (66756-1-Ig)

    IHC staining of human tonsillitis using 66756-1-Ig (same clone as 66756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66756-1-Ig (TRAIL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66756-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TRAIL Monoclonal antibody (66756-1-Ig)

    IHC staining of human tonsillitis using 66756-1-Ig (same clone as 66756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66756-1-Ig (TRAIL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66756-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TRAIL Monoclonal antibody (66756-1-Ig)

    IHC staining of human prostate cancer using 66756-1-Ig (same clone as 66756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66756-1-Ig (TRAIL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66756-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TRAIL Monoclonal antibody (66756-1-Ig)

    IHC staining of human prostate cancer using 66756-1-Ig (same clone as 66756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66756-1-Ig (TRAIL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66756-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50341-1

    Cytometric bead array standard curve of MP50341-1

    Cytometric bead array standard curve of MP50341-1, TRAIL Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66756-1-PBS. Detection antibody: 66756-2-PBS. Standard:Ag25746. Range: 3.125-200 ng/mL.

Product Information

66756-1-PBS targets TRAIL as part of a matched antibody pair:

MP50341-1: 66756-1-PBS capture and 66756-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRAIL fusion protein Ag25746
Full Name tumor necrosis factor (ligand) superfamily, member 10
Calculated Molecular Weight 281 aa, 33 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC032722
Gene Symbol TRAIL
Gene ID (NCBI) 8743
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TNFSF10/TRAIL (tumor necrosis factor superfamily member 10) is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TNFSF10 induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.