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PLA2G4D Monoclonal antibody

PLA2G4D Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E2A10

Cat no : 66752-1-Ig

Synonyms

cPLA2 delta, cPLA2delta, Phospholipase A2 group IVD, PLA2G4D

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLA2G4D Monoclonal antibody (66752-1-Ig)

    WB analysis using 66752-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66752-1-Ig (PLA2G4D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Caco-2 cells using PLA2G4D Monoclonal antibody (66752-1-Ig)

    WB analysis of Caco-2 using 66752-1-Ig

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 66752-1-Ig (PLA2G4D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PLA2G4D Monoclonal antibody (66752-1-Ig)

    IF Staining of HepG2 using 66752-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PLA2G4D antibody (66752-1-Ig, Clone: 2E2A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, L02 cells, SMMC-7721 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66752-1-Ig targets PLA2G4D in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLA2G4D fusion protein Ag4178
Full Name phospholipase A2, group IVD (cytosolic)
Calculated Molecular Weight 818 aa, 92 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberBC034571
Gene Symbol PLA2G4D
Gene ID (NCBI) 283748
RRIDAB_2882098
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLA2G4D(Cytosolic phospholipase A2 delta) belongs to the phospholipase A2 enzyme family,catalyzes the hydrolysis of glycerophospholipids at the sn-2 position and then liberate free fatty acids and lysophospholipids.PLA2G4D expression is specifically upregulated in the upper epidermis of patients with inflammatory skin diseases(PMID:14709560).

Protocols

Product Specific Protocols
WB protocol for PLA2G4D antibody 66752-1-IgDownload protocol
IF protocol for PLA2G4D antibody 66752-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols