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TSH Beta Monoclonal antibody

TSH Beta Monoclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2G1H3

Cat no : 66750-1-Ig

Synonyms

CHNG4, Thyrotropin alfa, Thyrotropin beta chain, Thyrotropin subunit beta, TSH B, TSH BETA, TSHB

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human pituitary tissue using TSH Beta Monoclonal antibody (66750-1-Ig)

    IHC staining of human pituitary using 66750-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66750-1-Ig (TSH beta antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pituitary tissue using TSH Beta Monoclonal antibody (66750-1-Ig)

    IHC staining of human pituitary using 66750-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66750-1-Ig (TSH beta antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human pituitary tissue using TSH Beta Monoclonal antibody (66750-1-Ig)

    IF Staining of human pituitary using 66750-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human pituitary tissue using TSH Beta antibody (66750-1-Ig, Clone: 2G1H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human pituitary tissue using TSH Beta Monoclonal antibody (66750-1-Ig)

    IF Staining of human pituitary using 66750-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human pituitary tissue using TSH Beta antibody (66750-1-Ig, Clone: 2G1H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive IHC detected inhuman pituitary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman pituitary tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:3000-1:12000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66750-1-Ig targets TSH Beta in IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TSH Beta fusion protein Ag27153
Full Name thyroid stimulating hormone, beta
Calculated Molecular Weight 138 aa, 16 kDa
GenBank Accession NumberBC069298
Gene Symbol TSH beta
Gene ID (NCBI) 7252
RRIDAB_2882097
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for TSH Beta antibody 66750-1-IgDownload protocol
IF protocol for TSH Beta antibody 66750-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Cancer Commun (Lond)

Local TSH/TSHR signaling promotes CD8+ T cell exhaustion and immune evasion in colorectal carcinoma

Authors - Sisi Zeng
View Article