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RBX1 Monoclonal antibody

RBX1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1F4D9

Cat no : 66716-1-Ig

Synonyms

BA554C12.1, Protein ZYP, RBX1, Regulator of cullins 1, ring box 1, RING box protein 1, RING finger protein 75, RNF75, ROC1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RBX1 Monoclonal antibody (66716-1-Ig)

    WB analysis using 66716-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66716-1-Ig (RBX1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using RBX1 Monoclonal antibody (66716-1-Ig)

    WB analysis of RAW 264.7 using 66716-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66716-1-Ig (RBX1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RBX1 Monoclonal antibody (66716-1-Ig)

    IHC staining of human liver cancer using 66716-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66716-1-Ig (RBX1 antibody) at dilution of 1:5000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RBX1 Monoclonal antibody (66716-1-Ig)

    IHC staining of human liver cancer using 66716-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66716-1-Ig (RBX1 antibody) at dilution of 1:5000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

Tested Applications

Positive WB detected inHeLa cells, RAW 264.7 cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66716-1-Ig targets RBX1 in WB, IHC, IP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RBX1 fusion protein Ag7005
Full Name ring-box 1
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 12-15 kDa
GenBank Accession NumberBC001466
Gene Symbol RBX1
Gene ID (NCBI) 9978
RRIDAB_2882067
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBX1(RING-box protein 1) is also named as RNF75, ROC1 and is a requisite component of the multi- subunit SCF family E3s36-40. It mediates the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair.

Protocols

Product Specific Protocols
WB protocol for RBX1 antibody 66716-1-IgDownload protocol
IHC protocol for RBX1 antibody 66716-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

The herpesvirus UL49.5 protein hijacks a cellular C-degron pathway to drive TAP transporter degradation

Authors - Magda Wąchalska
View Article
humanWB,IP

bioRxiv

The herpesvirus UL49.5 protein hijacks a cellular C-degron pathway to drive TAP transporter degradation

Authors - Magda Wąchalska
View Article