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TDG Monoclonal antibody

TDG Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G2E2

Cat no : 66707-1-Ig

Synonyms

TDG, thymine DNA glycosylase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TDG Monoclonal antibody (66707-1-Ig)

    WB analysis using 66707-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66707-1-Ig (TDG antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TDG Monoclonal antibody (66707-1-Ig)

    IHC staining of human breast cancer using 66707-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66707-1-Ig (TDG antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TDG Monoclonal antibody (66707-1-Ig)

    IHC staining of human breast cancer using 66707-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66707-1-Ig (TDG antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TDG Monoclonal antibody (66707-1-Ig)

    IF Staining of HepG2 using 66707-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66707-1-Ig (TDG antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HT-29 cells, COLO 320 cells, Jurkat cells, Raji cells, HSC-T6 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66707-1-Ig targets TDG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TDG fusion protein Ag27408
Full Name thymine-DNA glycosylase
Calculated Molecular Weight 410 aa, 46 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC037557
Gene Symbol TDG
Gene ID (NCBI) 6996
RRIDAB_2882059
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TDG belongs to the TDG/mug DNA glycosylase family. TDG corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. RNF4 interacts with and requires the base excision repair enzymes TDG and APE1 for active demethylation (PMID:20696907). TDG is modified by SUMO-1 and SUMO-2/3.The molecular weight of non-modified TDG is 46 kDa and modified TDG is 55-60 kDa.

Protocols

Product Specific Protocols
WB protocol for TDG antibody 66707-1-IgDownload protocol
IHC protocol for TDG antibody 66707-1-IgDownload protocol
IF protocol for TDG antibody 66707-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols