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FKBP8 Monoclonal antibody

FKBP8 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse and More (1)

Applications

WB, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1C1G12

Cat no : 66690-1-Ig

Synonyms

38 kDa FK506 binding protein, 38 kDa FKBP, FK506 binding protein 8, FK506 binding protein 8, 38kDa, FKBP 38, FKBP 8, FKBP38, FKBP8, FKBPr38, hFKBP38, PPIase FKBP8, Rotamase

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using FKBP8 Monoclonal antibody (66690-1-Ig)

    WB analysis of Jurkat using 66690-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FKBP8 Monoclonal antibody (66690-1-Ig)

    WB analysis using 66690-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FKBP8 Monoclonal antibody (66690-1-Ig)

    WB analysis of HeLa using 66690-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using FKBP8 Monoclonal antibody (66690-1-Ig)

    WB analysis of HEK-293 using 66690-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using FKBP8 Monoclonal antibody (66690-1-Ig)

    WB analysis of HepG2 using 66690-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using FKBP8 Monoclonal antibody (66690-1-Ig)

    WB analysis of NIH/3T3 using 66690-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using FKBP8 Monoclonal antibody (66690-1-Ig)

    WB analysis of RAW 264.7 using 66690-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using FKBP8 Monoclonal antibody (66690-1-Ig)

    IF Staining of A431 using 66690-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FKBP8 antibody (66690-1-Ig, Clone: 1C1G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, HepG2 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

CoIPSee 1 publications below

Product Information

66690-1-Ig targets FKBP8 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FKBP8 fusion protein Ag1649
Full Name FK506 binding protein 8, 38kDa
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC009966
Gene Symbol FKBP8
Gene ID (NCBI) 23770
RRIDAB_2882044
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FK506 binding protein 8 (FKBP8), is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP8 does not seem to have PPIase/rotamase activity, which is different from other members of this family. The active form of FKBP8 may play a role in the regulation of apoptosis. FKBP8 has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for FKBP8 antibody 66690-1-IgDownload protocol
IF protocol for FKBP8 antibody 66690-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCoIP

J Biol Chem

Lysine demethylase KDM1A promotes cell growth via FKBP8-BCL2 axis in hepatocellular carcinoma

Authors - Suli Lv
View Article