- Featured Product
- KD/KO Validated
IRAK1 Monoclonal antibody
IRAK1 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1H10A7
Cat no : 66653-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
| IF | See 3 publications below |
Product Information
66653-1-Ig targets IRAK1 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | IRAK1 fusion protein Ag0728 |
| Full Name | interleukin-1 receptor-associated kinase 1 |
| Calculated Molecular Weight | 77 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC014963 |
| Gene Symbol | IRAK1 |
| Gene ID (NCBI) | 3654 |
| RRID | AB_2882010 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Interleukin-1 receptor-associated kinases (IRAKs) are a unique family of death domain containing protein kinases that play a key role in initiating innate immune response against foreign pathogens. They are involved in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. IRAK1 is the first member of this kinase family. Upon ligand binding to TLR/IL-1R, IRAK1 is recruited by MYD88 to the receptor-signaling complex, the association leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Hyper-phosphorylated IRAK1 then disengages from the receptor complex, and forms a cytosolic IRAK1-TRAF6 complex. TRAF6 then interacts with TAK and TAB, resulting in eventual activation of the NF-κB and MAPK pathways. Phosphorylated IRAK1 also undergoes ubiquitin-mediated degradation or sumoylation, which results in nuclear translocation and transcriptional activation of inflammatory target genes. (PMID: 17890055; 12620219)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IRAK1 antibody 66653-1-Ig | Download protocol |
| IHC protocol for IRAK1 antibody 66653-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Mol Immunol Virus-inducible IGFALS facilitates innate immune responses by mediating IRAK1 and TRAF6 activation. | ||
Cell Death Dis Radiation induces IRAK1 expression to promote radioresistance by suppressing autophagic cell death via decreasing the ubiquitination of PRDX1 in glioma cells | ||
Biol Reprod miR-146b-5p downregulates IRAK1 and ADAM19 to suppress trophoblast proliferation, invasion and migration in miscarriage | ||
J Cell Biol BLK positively regulates TLR/IL-1R signaling by catalyzing TOLLIP phosphorylation |
