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ANGPTL8/Betatrophin Monoclonal antibody

ANGPTL8/Betatrophin Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2H9F9

Cat no : 66641-1-Ig

Synonyms

Angiopoietin-like protein 8, ANGPTL8, Betatrophin, C19orf80, Lipasin, LOC55908, PRO1185, PVPA599, td26

Validation Data Gallery

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  • Western Blot (WB) analysis of human adipose tissue using ANGPTL8/Betatrophin Monoclonal antibody (66641-1-Ig)

    WB analysis of human adipose using 66641-1-Ig

    human adipose tissue were subjected to SDS PAGE followed by western blot with 66641-1-Ig (ANGPTL8/Betatrophin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using ANGPTL8/Betatrophin Monoclonal antibody (66641-1-Ig)

    IHC staining of human liver using 66641-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66641-1-Ig (ANGPTL8/Betatrophin antibody) at dilution of 1:400 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ANGPTL8/Betatrophin Monoclonal antibody (66641-1-Ig)

    IHC staining of human liver using 66641-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66641-1-Ig (ANGPTL8/Betatrophin antibody) at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ANGPTL8/Betatrophin Monoclonal antibody (66641-1-Ig)

    IHC staining of human liver cancer using 66641-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66641-1-Ig (ANGPTL8/Betatrophin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ANGPTL8/Betatrophin Monoclonal antibody (66641-1-Ig)

    IHC staining of human liver cancer using 66641-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66641-1-Ig (ANGPTL8/Betatrophin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using ANGPTL8/Betatrophin Monoclonal antibody (66641-1-Ig)

    IF Staining of human liver using 66641-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using ANGPTL8/Betatrophin antibody (66641-1-Ig, Clone: 2H9F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman adipose tissue
Positive IHC detected inhuman liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66641-1-Ig targets ANGPTL8/Betatrophin in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ANGPTL8/Betatrophin fusion protein Ag20708
Full Name hepatocellular carcinoma-associated gene TD26
Calculated Molecular Weight 251 aa, 28 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC146552
Gene Symbol ANGPTL8
Gene ID (NCBI) 55908
RRIDAB_2882000
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ANGPTL8/Betatrophin antibody 66641-1-IgDownload protocol
IHC protocol for ANGPTL8/Betatrophin antibody 66641-1-IgDownload protocol
IF protocol for ANGPTL8/Betatrophin antibody 66641-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Br J Pharmacol

Sodium rutin extends lifespan and health span in mice including positive impacts on liver health.

Authors - Shuoshuo Li
View Article