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NEK2 Monoclonal antibody

NEK2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat and More (2)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

3D7B4

Cat no : 66632-1-Ig

Synonyms

HSPK 21, HsPK21, NEK2, NEK2A, NimA like protein kinase 1, NimA related protein kinase 2, NLK1

Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood leukocyte using NEK2 Monoclonal antibody (66632-1-Ig)

    WB analysis of human peripheral blood leukocyte using 66632-1-Ig

    Human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using NEK2 Monoclonal antibody (66632-1-Ig)

    WB analysis of THP-1 using 66632-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using NEK2 Monoclonal antibody (66632-1-Ig)

    WB analysis of K-562 using 66632-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using NEK2 Monoclonal antibody (66632-1-Ig)

    WB analysis of HL-60 using 66632-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using NEK2 Monoclonal antibody (66632-1-Ig)

    WB analysis of Jurkat using 66632-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using NEK2 Monoclonal antibody (66632-1-Ig)

    WB analysis of RAW 264.7 using 66632-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat thymus tissue using NEK2 Monoclonal antibody (66632-1-Ig)

    WB analysis of rat thymus using 66632-1-Ig

    rat thymus tissue were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using NEK2 Monoclonal antibody (66632-1-Ig)

    IHC staining of human testis using 66632-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66632-1-Ig (NEK2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using NEK2 Monoclonal antibody (66632-1-Ig)

    IHC staining of human testis using 66632-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66632-1-Ig (NEK2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using NEK2 Monoclonal antibody (66632-1-Ig)

    IF Staining of A431 using 66632-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NEK2 antibody (66632-1-Ig, Clone: 3D7B4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

Tested Applications

Positive WB detected inHuman peripheral blood leukocyte, THP-1 cells, K-562 cells, HL-60 cells, Jurkat cells, RAW 264.7 cells, rat thymus tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66632-1-Ig targets NEK2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Reactivity Human, Mouse , Rat
Cited Reactivityhuman, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NEK2 fusion protein Ag21476
Full Name NIMA (never in mitosis gene a)-related kinase 2
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC043502
Gene Symbol NEK2
Gene ID (NCBI) 4751
RRIDAB_2881991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NEK2 antibody 66632-1-IgDownload protocol
IHC protocol for NEK2 antibody 66632-1-IgDownload protocol
IF protocol for NEK2 antibody 66632-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Toxicol Lett

Oxcarbazepine induces mitotic catastrophe and apoptosis in NRK-52E proximal tubular cells.

Authors - Momoka Ota
View Article
humanWB,IF,CoIP

Cancer Cell Int

NEK2 contributes to radioresistance in esophageal squamous cell carcinoma by inducing protective autophagy via regulating TRIM21

Authors - Dong Guo
  • KD Validated
View Article