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CHCHD5 Monoclonal antibody

CHCHD5 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2G10A7

Cat no : 66584-1-Ig

Synonyms

C2orf9, CHCHD5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHCHD5 Monoclonal antibody (66584-1-Ig)

    WB analysis using 66584-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66584-1-Ig (CHCHD5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HeLa cells using CHCHD5 Monoclonal antibody (66584-1-Ig)

    WB analysis of HeLa using 66584-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66584-1-Ig (CHCHD5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CHCHD5 Monoclonal antibody (66584-1-Ig)

    IHC staining of human ovary tumor using 66584-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66584-1-Ig (CHCHD5 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CHCHD5 Monoclonal antibody (66584-1-Ig)

    IHC staining of human ovary tumor using 66584-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66584-1-Ig (CHCHD5 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, THP-1 cells, HL-60 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66584-1-Ig targets CHCHD5 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CHCHD5 fusion protein Ag22534
Full Name coiled-coil-helix-coiled-coil-helix domain containing 5
Calculated Molecular Weight 110 aa, 12 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC004498
Gene Symbol CHCHD5
Gene ID (NCBI) 84269
RRIDAB_2881944
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CHCHD5 antibody 66584-1-IgDownload protocol
IHC protocol for CHCHD5 antibody 66584-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols