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  • Featured Product
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YME1L1 Monoclonal antibody

YME1L1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B7E4

Cat no : 66551-1-Ig

Synonyms

FTSH, FTSH1, Meg 4, MEG4, PAMP, YME1 like 1 (S. cerevisiae), YME1 like protein 1, YME1L, YME1L1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using YME1L1 Monoclonal antibody (66551-1-Ig)

    WB analysis of HeLa using 66551-1-Ig

    WB result of YME1L1 antibody (66551-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YME1L1 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using YME1L1 Monoclonal antibody (66551-1-Ig)

    WB analysis using 66551-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66551-1-Ig (YME1L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of NIH/3T3 cells using YME1L1 Monoclonal antibody (66551-1-Ig)

    WB analysis of NIH/3T3 using 66551-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66551-1-Ig (YME1L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using YME1L1 Monoclonal antibody (66551-1-Ig)

    IHC staining of human kidney using 66551-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66551-1-Ig (YME1L1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using YME1L1 Monoclonal antibody (66551-1-Ig)

    IHC staining of human kidney using 66551-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66551-1-Ig (YME1L1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, Raji cells, THP-1 cells, HSC-T6 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66551-1-Ig targets YME1L1 in WB, IHC, ELISA applications and shows reactivity with Human, Rat, mouse samples.

Tested Reactivity Human, Rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen YME1L1 fusion protein Ag2069
Full Name YME1-like 1 (S. cerevisiae)
Calculated Molecular Weight 716 aa, 80 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC023507
Gene Symbol YME1L1
Gene ID (NCBI) 10730
RRIDAB_2881913
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).

Protocols

Product Specific Protocols
WB protocol for YME1L1 antibody 66551-1-IgDownload protocol
IHC protocol for YME1L1 antibody 66551-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols