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Calponin Monoclonal antibody

Calponin Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A11E5

Cat no : 66540-1-Ig

Synonyms

Basic calponin, Calponin, Calponin 1, Calponin H1, smooth muscle, CNN1, Sm Calp, SMCC

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Calponin Monoclonal antibody (66540-1-Ig)

    WB analysis using 66540-1-Ig

    Various tissue were subjected to SDS PAGE followed by western blot with 66540-1-Ig (Calponin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human myofibroblastoma tissue using Calponin Monoclonal antibody (66540-1-Ig)

    IHC staining of human myofibroblastoma using 66540-1-Ig

    Immunohistochemical analysis of paraffin-embedded human myofibroblastoma tissue slide using 66540-1-Ig (Calponin antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human myofibroblastoma tissue using Calponin Monoclonal antibody (66540-1-Ig)

    IHC staining of human myofibroblastoma using 66540-1-Ig

    Immunohistochemical analysis of paraffin-embedded human myofibroblastoma tissue slide using 66540-1-Ig (Calponin antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman heart tissue, human vein tissue, human colon tissue, human ileum tissue, human bladder tissue, human kidney tissue
Positive IHC detected inhuman myofibroblastoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1500-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66540-1-Ig targets Calponin in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Calponin fusion protein Ag21384
Full Name calponin 1, basic, smooth muscle
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33-35 kDa
GenBank Accession NumberBC022015
Gene Symbol Calponin
Gene ID (NCBI) 1264
RRIDAB_2881902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the specific region of human CNN1, thus it does not cross-react with CNN2 or CNN3.

Protocols

Product Specific Protocols
WB protocol for Calponin antibody 66540-1-IgDownload protocol
IHC protocol for Calponin antibody 66540-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols