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Caspase 2/p32/p18 Monoclonal antibody, PBS Only (Detector)

Caspase 2/p32/p18 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G4C2

Cat no : 66517-1-PBS

Synonyms

CASP2, Caspase 2, Caspase 2/p18, CASP-2, CASP 2

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using Caspase 2/p32/p18 Monoclonal antibody (66517-1-Ig)

    WB analysis of Jurkat using 66517-1-Ig (same clone as 66517-1-PBS)

    Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66517-1-Ig (Caspase 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66517-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50435-1

    Cytometric bead array standard curve of MP50435-1

    Cytometric bead array standard curve of MP50435-1, Caspase 2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66517-2-PBS. Detection antibody: 66517-1-PBS. Standard:Ag20141. Range: 0.098-25 ng/mL.

Product Information

66517-1-PBS targets Caspase 2/p32/p18 as part of a matched antibody pair:

MP50435-1: 66517-2-PBS capture and 66517-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Caspase 2/p32/p18 fusion protein Ag20141
Full Name caspase 2, apoptosis-related cysteine peptidase
Calculated Molecular Weight 452 aa, 51 kDa
Observed Molecular Weight48-51 kDa, 32 kDa, 18 kDa
GenBank Accession NumberBC002427
Gene Symbol Caspase 2
Gene ID (NCBI) 835
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).This antibody can recognize the 32 kDa pro-caspase 2 as well as 18 kDa cleaved-caspase 2.