MDM2 Monoclonal antibody
MDM2 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, rat, rabbit and More (1)
Applications
WB, IHC, IF, IP, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
2A6C9
Cat no : 66511-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, A549 cells, mouse brain tissue, rabbit brain tissue, rat brain tissue, U-251 cells |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 23 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
| IP | See 2 publications below |
| CoIP | See 2 publications below |
Product Information
66511-1-Ig targets MDM2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.
| Tested Reactivity | Human, mouse, rat, rabbit |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | Mdm2 p53 binding protein homolog (mouse) |
| Calculated Molecular Weight | 55 kDa |
| Observed Molecular Weight | 40-55 kDa |
| GenBank Accession Number | NM_002392 |
| Gene Symbol | MDM2 |
| Gene ID (NCBI) | 4193 |
| RRID | AB_2881874 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
What is the cellular localization of MDM2?
MDM2 is predominantly localized in the nucleoplasm.
What is the tissue specificity of MDM2?
MDM2 is non-specific, expressing ubiquitously in the brain, heart, kidney, skin, and other tissues. Certain
isoforms only express in cancerous cells and not normal tissue.
With what other molecules does MDM2 interact?
Most notably, the oncogene protein MDM2 regulates transcription factor p53 by binding its transcriptional
activation domain and by E3-ubiquitin ligase activity. Ribosome proteins rpL5, rpL11, and rpL23 also bind to
MDM2, inhibiting E3 ubiquitin ligase activity and promoting the p53 pathway.
What competes with MDM2 activity?
Nutilin-3 was designed to compete with MDM2 in binding with p53, inducing activation of the p53 pathway.
What is the role of ubiquitination in MDM2 activity?
While p53 is also affected by other post-translational modifications, its stability is determined by ubiquitination
mediation of MDM2, suppressing p53 transcriptional regulation and leading to the proteosomal degradation of
p53.
How is MDM2 associated with cancer?
MDM2 inhibits the activity of the tumor-suppressive protein p53, which serves as an important factor in
promoting DNA repair or activating apoptotic pathways. Loss of p53 is associated with tumor development
and growth.
What is the human ortholog of MDM2?
Human double-minute type 2 (HDM2)
In what cellular circumstances does the p53-MDM2 complex dissociate?
Cellular stress or DNA damage result in the phosphorylation and activation of p53. Phosphorylation also
prevents p53-MDM2 interaction.
Where is the gene located?
Hdm2 is located on human 12q15.
Mdm2 is located on murine 10 D2.
PMIDs: 24309898, 29584707, 9840926, 28714398
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MDM2 antibody 66511-1-Ig | Download protocol |
| IHC protocol for MDM2 antibody 66511-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Redox Biol A new FGF1 variant protects against adriamycin-induced cardiotoxicity via modulating p53 activity. | ||
Cell Death Discov LASS2 enhances p53 protein stability and nuclear import to suppress liver cancer progression through interaction with MDM2/MDMX | ||
Cell Death Discov hMOF induces cisplatin resistance of ovarian cancer by regulating the stability and expression of MDM2 | ||
Oncogenesis MeCP2 facilitates breast cancer growth via promoting ubiquitination-mediated P53 degradation by inhibiting RPL5/RPL11 transcription. | ||
Int J Biol Sci Flap endonuclease 1 Facilitated Hepatocellular Carcinoma Progression by Enhancing USP7/MDM2-mediated P53 Inactivation. | ||
J Cell Mol Med LncRNA MIAT enhances cerebral ischaemia/reperfusion injury in rat model via interacting with EGLN2 and reduces its ubiquitin-mediated degradation. |
