Phospho-AKT (Ser473) Monoclonal antibody, PBS Only (Capture)
Phospho-AKT (Ser473) Monoclonal Antibody for WB, FC, IHC, Indirect ELISA, Cytometric bead array
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, FC, IHC, Indirect ELISA, Cytometric bead array
Conjugate
Unconjugated
CloneNo.
1C10B8
Cat no : 66444-1-PBS
Synonyms
Validation Data Gallery
Recommended dilution
Application | Dilution |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
Product Information
66444-1-PBS targets Phospho-AKT (Ser473) as part of a matched antibody pair:
MP50177-1: 66444-1-PBS capture and 60203-2-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | v-akt murine thymoma viral oncogene homolog 1 |
Observed Molecular Weight | 60-62 kDa |
GenBank Accession Number | NM_005163 |
Gene Symbol | AKT1 |
Gene ID (NCBI) | 207 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with phospho-modification at Ser473.