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CSN2 Monoclonal antibody

CSN2 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2H10E6

Cat no : 66435-1-Ig

Synonyms

ALIEN, Alien homolog, COPS2, CSN2, SGN2, Signalosome subunit 2, TR interacting protein 15, TRIP 15, TRIP15

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using CSN2 Monoclonal antibody (66435-1-Ig)

    WB analysis of NIH/3T3 using 66435-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66435-1-Ig (CSN2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of ROS1728 cells using CSN2 Monoclonal antibody (66435-1-Ig)

    WB analysis of ROS1728 using 66435-1-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66435-1-Ig (CSN2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using CSN2 Monoclonal antibody (66435-1-Ig)

    WB analysis of C6 using 66435-1-Ig

    C6 cells were subjected to SDS PAGE followed by western blot with 66435-1-Ig (CSN2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using CSN2 Monoclonal antibody (66435-1-Ig)

    WB analysis of RAW 264.7 using 66435-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66435-1-Ig (CSN2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CSN2 Monoclonal antibody (66435-1-Ig)

    IHC staining of human ovary tumor using 66435-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66435-1-Ig (CSN2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CSN2 Monoclonal antibody (66435-1-Ig)

    IHC staining of human ovary tumor using 66435-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66435-1-Ig (COPS2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human ovary tumor tissue using CSN2 Monoclonal antibody (66435-1-Ig)

    IF Staining of human ovary tumor using 66435-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CSN2 antibody (66435-1-Ig, Clone: 2H10E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inNIH/3T3 cells, C6 cells, RAW 264.7 cells, ROS1728 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman ovary tumor tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66435-1-Ig targets CSN2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CSN2 fusion protein Ag1415
Full Name COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis)
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC012629
Gene Symbol COPS2
Gene ID (NCBI) 9318
RRIDAB_2881805
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COPS2 is an essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.

Protocols

Product Specific Protocols
WB protocol for CSN2 antibody 66435-1-IgDownload protocol
IHC protocol for CSN2 antibody 66435-1-IgDownload protocol
IF protocol for CSN2 antibody 66435-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols