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PGRMC1 Monoclonal antibody

PGRMC1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1C10D11

Cat no : 66372-1-Ig

Synonyms

HPR6.6, MPR, PGRMC, PGRMC1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PGRMC1 Monoclonal antibody (66372-1-Ig)

    WB analysis of HEK-293 using 66372-1-Ig

    WB result of PGRMC1 antibody (66372-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PGRMC1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using PGRMC1 Monoclonal antibody (66372-1-Ig)

    WB analysis using 66372-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66372-1-Ig (PGRMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of fetal human brain tissue using PGRMC1 Monoclonal antibody (66372-1-Ig)

    WB analysis of fetal human brain using 66372-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66372-1-Ig (PGRMC1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PGRMC1 Monoclonal antibody (66372-1-Ig)

    WB analysis of MCF-7 using 66372-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66372-1-Ig (PGRMC1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using PGRMC1 Monoclonal antibody (66372-1-Ig)

    WB analysis of NIH/3T3 using 66372-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66372-1-Ig (PGRMC1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PGRMC1 Monoclonal antibody (66372-1-Ig)

    IHC staining of human ovary tumor using 66372-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66372-1-Ig (PGRMC1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PGRMC1 Monoclonal antibody (66372-1-Ig)

    IHC staining of human ovary tumor using 66372-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66372-1-Ig (PGRMC1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PGRMC1 Monoclonal antibody (66372-1-Ig)

    IHC staining of human lung cancer using 66372-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66372-1-Ig (PGRMC1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PGRMC1 Monoclonal antibody (66372-1-Ig)

    IHC staining of human lung cancer using 66372-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66372-1-Ig (PGRMC1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human ovary tumor tissue using PGRMC1 Monoclonal antibody (66372-1-Ig)

    IF Staining of human ovary tumor using 66372-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using PGRMC1 antibody (66372-1-Ig, Clone: 1C10D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human ovary tumor tissue using PGRMC1 Monoclonal antibody (66372-1-Ig)

    IF Staining of human ovary tumor using 66372-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using PGRMC1 antibody (66372-1-Ig, Clone: 1C10D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, fetal human brain tissue, MCF-7 cells, NIH/3T3 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman ovary tumor tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman ovary tumor tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66372-1-Ig targets PGRMC1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PGRMC1 fusion protein Ag3643
Full Name progesterone receptor membrane component 1
Calculated Molecular Weight 195 aa, 22 kDa
Observed Molecular Weight 25-27 kDa
GenBank Accession NumberBC034238
Gene Symbol PGRMC1
Gene ID (NCBI) 10857
RRIDAB_2881751
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Progesterone receptor membrane component 1 (PGRMC1) is a member of a multi-protein progesterone-binding complex. However, PGRMC1 shares homology with cytochrome b5-related proteins rather than hormone receptors (PMID: 18992768). It is a heme binding protein with biding sites for Src homology (SH2) and SH3 domain-containing proteins (PMID: 17583495). PGRMC1 is overexpressed in a variety of cancers, and thus represents an important biomarker for cancer progression and a potential target for anticancer drugs (PMID: 21730960). In nonmalignant tissues, PGRMC1 is highly expressed in the liver and kidney (PMID: 9705155; 20164297).

Protocols

Product Specific Protocols
WB protocol for PGRMC1 antibody 66372-1-IgDownload protocol
IHC protocol for PGRMC1 antibody 66372-1-IgDownload protocol
IF protocol for PGRMC1 antibody 66372-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF,WB

Biol Chem

A progesterone receptor membrane component 1 antagonist induces large vesicles independent of progesterone receptor membrane component 1 expression.

Authors - Lihua Wang-Eckhardt
View Article