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EBI3 Monoclonal antibody

EBI3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F3H10

Cat no : 66354-1-Ig

Synonyms

EBI3, EBV induced gene 3 protein, Epstein Barr virus induced 3, IL 27 subunit beta, IL 27B, IL27B, Interleukin 27 subunit beta

Validation Data Gallery

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  • Western Blot (WB) analysis of human spleen tissue using EBI3 Monoclonal antibody (66354-1-Ig)

    WB analysis of human spleen using 66354-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66354-1-Ig (EBI3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat spleen tissue using EBI3 Monoclonal antibody (66354-1-Ig)

    WB analysis of rat spleen using 66354-1-Ig

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 66354-1-Ig (EBI3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman spleen tissue, rat spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66354-1-Ig targets EBI3 in WB, ELISA applications and shows reactivity with human, pig, rat samples.

Tested Reactivity human, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EBI3 fusion protein Ag3051
Full Name Epstein-Barr virus induced 3
Calculated Molecular Weight 229 aa, 25 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC015364
Gene Symbol EBI3
Gene ID (NCBI) 10148
RRIDAB_2881734
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EBI3, a 34-kDa secreted glycoprotein, is a subunit in 2 distinct heterodimeric cytokines: IL-27 and IL-35. IL-27, composed of p28 (IL-27) and EBI3, can trigger signaling in T cells, B cells, and myeloid cells. IL-35, composed of EBI3 and the ubiquitously expressed p35 subunit of IL-12, is an inhibitory cytokine involved in regulatory T-cell function. EBI3 plays a role in regulating cell-mediated immune responses. (PMID: 8551575; 18033300; 17874423; 21931777)

Protocols

Product Specific Protocols
WB protocol for EBI3 antibody 66354-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols