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- KD/KO Validated
5 Lipoxygenase Monoclonal antibody
5 Lipoxygenase Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, pig
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
3B1B7
Cat no : 66326-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | pig spleen tissue, THP-1 cells |
| Positive IHC detected in | human lung tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
66326-1-Ig targets 5 Lipoxygenase in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | 5 Lipoxygenase fusion protein Ag18168 |
| Full Name | arachidonate 5-lipoxygenase |
| Calculated Molecular Weight | 674 aa, 78 kDa |
| Observed Molecular Weight | 70-78 kDa |
| GenBank Accession Number | BC130332 |
| Gene Symbol | 5 Lipoxygenase |
| Gene ID (NCBI) | 240 |
| RRID | AB_2881707 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for 5 Lipoxygenase antibody 66326-1-Ig | Download protocol |
| IHC protocol for 5 Lipoxygenase antibody 66326-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Gene Ther Knock-out of 5-lipoxygenase in overexpressing tumor cells-consequences on gene expression and cellular function | ||
Life Sci Alliance Three-dimensional growth reveals fine-tuning of 5-lipoxygenase by proliferative pathways in cancer | ||
Kaohsiung J Med Sci Magnolin alleviated DSS-induced colitis by inhibiting ALOX5-mediated ferroptosis | ||
Acta Diabetol Identification of potential key lipid metabolism-related genes involved in tubular injury in diabetic kidney disease by bioinformatics analysis | ||
Cell Death Discov Crizotinib and its enantiomer suppress ferroptosis by decreasing PE-O-PUFA content |
