Filter:
Tested Applications
| Positive WB detected in | K-562 cells |
| Positive IF/ICC detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
66246-1-Ig targets BBS2 in WB, IF/ICC, ELISA applications and shows reactivity with human, Canine samples.
| Tested Reactivity | human, Canine |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21390 Product name: Recombinant human BBS2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-325 aa of BC014140 Sequence: MLLPVFTLKLRHKISPRMVAIGRYDGTHPCLAAATQTGKVFIHNPHTRNQHVSASRVFQSPLESDVSLLNINQAVSCLTAGVLNPELGYDALLVGTQTNLLAYDVYNNSDLFYREVADGANVVVLGTLGDISSPLAIIGGNCALQGFNHEGSDLFWTVTGDNVNSLALCDFDGDGKKELLVGSEDFDIRVFKEDEIVAEMTETEIVTSLCPMYGSRFGYALSNGTVGVYDKTSRYWRIKSKNHAMSIHAFDLNSDGVNELITGWSNGKVDARSDRTGEVIFKDNFSSAIAGVVEGDYRMDGHIQLICCSVDGEIRGYLPGTAEMR Predict reactive species |
| Full Name | Bardet-Biedl syndrome 2 |
| Calculated Molecular Weight | 80 kDa |
| Observed Molecular Weight | 80-90 kDa |
| GenBank Accession Number | BC014140 |
| Gene Symbol | BBS2 |
| Gene ID (NCBI) | 583 |
| RRID | AB_2881635 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BXC9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for BBS2 antibody 66246-1-Ig | Download protocol |
| WB protocol for BBS2 antibody 66246-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Proteomics CRISPR/Cas9-mediated genomic editing of Cluap1/IFT38 reveals a new role in actin arrangement. | ||
J Biol Chem The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors. |
