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S100A10 Monoclonal antibody

S100A10 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B3F9

Cat no : 66227-1-Ig

Synonyms

42C, ANX2L, ANX2LG, Ca[1], CAL1L, Calpactin 1 light chain, Calpactin I light chain, Cellular ligand of annexin II, CLP11, GP11, p10, p10 protein, P11, Protein S100 A10, S100A10

Validation Data Gallery

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  • Western Blot (WB) analysis of human lung tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    WB analysis of human lung using 66227-1-Ig

    human lung tissue were subjected to SDS PAGE followed by western blot with 66227-1-Ig (S100A10 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    IHC staining of human colon cancer using 66227-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66227-1-Ig (S100A10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    IHC staining of human colon cancer using 66227-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66227-1-Ig (S100A10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    IHC staining of human stomach cancer using 66227-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    IHC staining of human liver cancer using 66227-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    IHC staining of human liver cancer using 66227-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    IHC staining of human cervical cancer using 66227-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    IHC staining of human cervical cancer using 66227-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    IHC staining of human colon cancer using 66227-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    IHC staining of human pancreas cancer using 66227-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    IHC staining of human pancreas cancer using 66227-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66227-1-Ig (S100A10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    IHC staining of human pancreas cancer using 66227-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66227-1-Ig (S100A10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using S100A10 Monoclonal antibody (66227-1-Ig)

    IF Staining of A431 using 66227-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using S100A10 antibody (66227-1-Ig, Clone: 1B3F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of Saos-2 cells using S100A10 Monoclonal antibody (66227-1-Ig)

    IF Staining of Saos-2 using 66227-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using S100A10 antibody (66227-1-Ig, Clone: 1B3F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman lung tissue
Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue, human liver cancer tissue, human stomach cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, Saos-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

66227-1-Ig targets S100A10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen S100A10 fusion protein Ag23596
Full Name S100 calcium binding protein A10
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 11 kDa
GenBank Accession NumberBC015973
Gene Symbol S100A10
Gene ID (NCBI) 6281
RRIDAB_2881618
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.

Protocols

Product Specific Protocols
WB protocol for S100A10 antibody 66227-1-IgDownload protocol
IHC protocol for S100A10 antibody 66227-1-IgDownload protocol
IF protocol for S100A10 antibody 66227-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Exp Neurol

Voluntary wheel exercise improves glymphatic clearance and ameliorates colitis-associated cognitive impairment in aged mice by inhibiting TRPV4-induced astrocytic calcium activity

Authors - Mingyue Li
View Article