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  • Featured Product
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USP5 Monoclonal antibody

USP5 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

3F12C10

Cat no : 66213-1-Ig

Synonyms

ISOT 1, ISOT, Isopeptidase T, EC:3.4.19.12, Deubiquitinating enzyme 5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP5 Monoclonal antibody (66213-1-Ig)

    WB analysis using 66213-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of Jurkat cells using USP5 Monoclonal antibody (66213-1-Ig)

    WB analysis of Jurkat using 66213-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using USP5 Monoclonal antibody (66213-1-Ig)

    WB analysis of A549 using 66213-1-Ig

    WB result of USP5 antibody (66213-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP5 transfected A549 cells.

  • Western Blot (WB) analysis of HeLa cells using USP5 Monoclonal antibody (66213-1-Ig)

    WB analysis of HeLa using 66213-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using USP5 Monoclonal antibody (66213-1-Ig)

    WB analysis of HeLa using 66213-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using USP5 Monoclonal antibody (66213-1-Ig)

    WB analysis of HEK-293 using 66213-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using USP5 Monoclonal antibody (66213-1-Ig)

    IF Staining of A549 using 66213-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using USP5 antibody (66213-1-Ig, Clone: 3F12C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inU2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, A549 cells, LNCaP cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66213-1-Ig targets USP5 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen USP5 fusion protein Ag7575
Full Name ubiquitin specific peptidase 5 (isopeptidase T)
Calculated Molecular Weight 96 kDa
Observed Molecular Weight 95-105 kDa
GenBank Accession NumberBC005139
Gene Symbol USP5
Gene ID (NCBI) 8078
RRIDAB_2881604
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers.

Protocols

Product Specific Protocols
WB protocol for USP5 antibody 66213-1-IgDownload protocol
IF protocol for USP5 antibody 66213-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy

Authors - Xiangling Xiao
View Article
human,mouseIF,CoIP

Cell Commun Signal

Deubiquitinase USP5 regulates RIPK1 driven pyroptosis in response to myocardial ischemic reperfusion injury

Authors - Wenjing Sun
View Article
ratWB

Sci Rep

Dynamic recruitment of ubiquitin to mutant huntingtin inclusion bodies.

Authors - Katrin Juenemann
View Article