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Septin 8 Monoclonal antibody

Septin 8 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4A12G9

Cat no : 66188-1-Ig

Synonyms

KIAA0202, SEP2, SEPT8, septin 8, septin8

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Septin 8 Monoclonal antibody (66188-1-Ig)

    WB analysis of HeLa using 66188-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66188-1-Ig (Septin 8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Septin 8 Monoclonal antibody (66188-1-Ig)

    IHC staining of mouse brain using 66188-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66188-1-Ig (Septin 8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Septin 8 Monoclonal antibody (66188-1-Ig)

    IHC staining of mouse brain using 66188-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66188-1-Ig (Septin 8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Septin 8 Monoclonal antibody (66188-1-Ig)

    IHC staining of human gliomas using 66188-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66188-1-Ig (Septin 8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Septin 8 Monoclonal antibody (66188-1-Ig)

    IHC staining of human gliomas using 66188-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66188-1-Ig (Septin 8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Septin 8 Monoclonal antibody (66188-1-Ig)

    IF Staining of HeLa using 66188-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Septin 8 antibody (66188-1-Ig, Clone: 4A12G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Septin 8 Monoclonal antibody (66188-1-Ig)

    IF Staining of HeLa using 66188-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Septin 8 antibody (66188-1-Ig, Clone: 4A12G9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inmouse brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66188-1-Ig targets Septin 8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen Septin 8 fusion protein Ag19114
Full Name septin 8
Calculated Molecular Weight55.6 kDa
Observed Molecular Weight 50-58 kDa
GenBank Accession NumberBC001329
Gene Symbol Septin 8
Gene ID (NCBI) 23176
RRIDAB_2881583
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Septin 8, also named as KIAA0202, is a member of the highly conserved septin family. Septins are membrane-associated GTPases which are involved in cytokinesis and cellular morphogenesis. Septin 8 is an interaction partner of Septin 4. It may play a role in platelet secretion.

Protocols

Product Specific Protocols
WB protocol for Septin 8 antibody 66188-1-IgDownload protocol
IHC protocol for Septin 8 antibody 66188-1-IgDownload protocol
IF protocol for Septin 8 antibody 66188-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols