IFN Alpha Monoclonal antibody

IFN Alpha Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4A2A11

Cat no : 66162-1-Ig

Synonyms

IFL, IFN, IFN ALPHA, IFN alpha 1/13, IFNA@, IFNA1, IFNA13, IFN-alpha, LeIF D



Tested Applications

Positive WB detected inRecombinant protein, Transfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

66162-1-Ig targets IFN Alpha in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IFN Alpha fusion protein Ag12533
Full Name IFNA1
Calculated Molecular Weight 189 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC074928
Gene Symbol IFN alpha1
Gene ID (NCBI) 3439
RRIDAB_2881558
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFNA1 (IF-alpha) is a member of the Type I IFN (1) family best known for their antiviral activity. It is a key cytokine regulating the activity of B cells, T-helper cells (Th cells), cDCs and natural killer cells (NK cells). IFNA1 induces B cell maturation into plasma cells and immunoglobulin production. IFNA1 plays an important role in the pathogenesis of systemic lupus erythematosus (SLE). IFNA1 was the first cytokine to show clinical benefit in the treatment of certain types of cancer, including melanoma, chronic myelogenous leukemia, and renal cancer.

Protocols

Product Specific Protocols
WB protocol for IFN Alpha antibody 66162-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Amino Acids

Escherichia coli infection activates the production of IFN-α and IFN-β via the JAK1/STAT1/2 signaling pathway in lung cells.

Authors - Yan Jin
humanWB

Brain Res

Serum miR-221-3p as a new potential biomarker for depressed mood in perioperative patients.

Authors - Jianguo Feng
humanWB

Cell Rep

Nuclear translocation of cGAS orchestrates VEGF-A-mediated angiogenesis

Authors - Juanjuan Luo