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RAC1 Monoclonal antibody

RAC1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, PLA

Conjugate

Unconjugated

CloneNo.

4A4B11

Cat no : 66122-1-Ig

Synonyms

MIG5, p21 Rac1, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAC1 Monoclonal antibody (66122-1-Ig)

    WB analysis using 66122-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66122-1-Ig (Rac1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RAC1 Monoclonal antibody (66122-1-Ig)

    WB analysis of NIH/3T3 using 66122-1-Ig

    WB result of RAC1 antibody (66122-1-Ig, 1:2000) with si-control and si-RAC1 transfected NIH3T3 cells.

  • Western Blot (WB) analysis of human brain tissue using RAC1 Monoclonal antibody (66122-1-Ig)

    WB analysis of human brain using 66122-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66122-1-Ig (RAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RAC1 Monoclonal antibody (66122-1-Ig)

    WB analysis of HeLa using 66122-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66122-1-Ig (Rac1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HL-60 cells using RAC1 Monoclonal antibody (66122-1-Ig)

    IP experiment of HL-60 using 66122-1-Ig

    IP result of anti-RAC1 (IP:66122-1-Ig, 3ug; Detection:66122-1-Ig 1:700) with HL-60 cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAC1 Monoclonal antibody (66122-1-Ig)

    IHC staining of human colon cancer using 66122-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66122-1-Ig (RAC1 Antibody) at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAC1 Monoclonal antibody (66122-1-Ig)

    IHC staining of human colon cancer using 66122-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66122-1-Ig (RAC1 Antibody) at dilution of 1:400 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using RAC1 Monoclonal antibody (66122-1-Ig)

    IF Staining of NIH/3T3 using 66122-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed NIH/3T3 cells using 66122-1-Ig(Rac1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAC1 Monoclonal antibody (66122-1-Ig)

    IF Staining of HeLa using 66122-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66122-1-Ig(Rac1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue, NIH/3T3 cells, HeLa cells, HUVEC cells, HL-60 cells, HSC-T6 cells, RAW 264.7 cells
Positive IP detected inHL-60 cells, HSC-T6 cells, Raw 264.7 cells, HUVEC cells, HL-60 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66122-1-Ig targets RAC1 in WB, IHC, IF/ICC, IP, CoIP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RAC1 fusion protein Ag17779
Full Name ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC004247
Gene Symbol RAC1
Gene ID (NCBI) 5879
RRIDAB_2881521
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.

Protocols

Product Specific Protocols
WB protocol for RAC1 antibody 66122-1-IgDownload protocol
IHC protocol for RAC1 antibody 66122-1-IgDownload protocol
IF protocol for RAC1 antibody 66122-1-IgDownload protocol
IP protocol for RAC1 antibody 66122-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

TCR activation directly stimulates PYGB-dependent glycogenolysis to fuel the early recall response in CD8+ memory T cells.

Authors - Huafeng Zhang
View Article
humanPLA

Nat Commun

Regulation of local GTP availability controls RAC1 activity and cell invasion

Authors - Anna Bianchi-Smiraglia
View Article
mouseWB

Nat Commun

TLR4 signalling via Piezo1 engages and enhances the macrophage mediated host response during bacterial infection.

Authors - Jing Geng
View Article
mouseWB

Circ. Res.

Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.

Authors - Dong Ma
View Article
humanWB

Oncogene

Smoking-associated upregulation of CBX3 suppresses ARHGAP24 expression to activate Rac1 signaling and promote tumor progression in lung adenocarcinoma.

Authors - Xin Jin
View Article
humanWB

Cell Chem Biol

Phosphorylation of guanosine monophosphate reductase triggers a GTP-dependent switch from pro- to anti-oncogenic function of EPHA4.

Authors - David W Wolff
View Article