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TELO2 Monoclonal antibody

TELO2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

3H10F4

Cat no : 66077-1-Ig

Synonyms

hCLK2, KIAA0683, Protein clk 2 homolog, TEL2, TELO2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TELO2 Monoclonal antibody (66077-1-Ig)

    WB analysis using 66077-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66077-1-Ig (TELO2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using TELO2 Monoclonal antibody (66077-1-Ig)

    WB analysis of Y79 using 66077-1-Ig

    Y79 cells were subjected to SDS PAGE followed by western blot with 66077-1-Ig (TELO2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using TELO2 Monoclonal antibody (66077-1-Ig)

    WB analysis of L02 using 66077-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 66077-1-Ig (TELO2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using TELO2 Monoclonal antibody (66077-1-Ig)

    WB analysis of A431 using 66077-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 66077-1-Ig (TELO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using TELO2 Monoclonal antibody (66077-1-Ig)

    IP experiment of HepG2 using 66077-1-Ig

    IP result of anti-TELO2 (IP:66077-1-Ig, 4ug; Detection:66077-1-Ig 1:500) with HepG2 cells lysate 2900ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TELO2 Monoclonal antibody (66077-1-Ig)

    IHC staining of human kidney using 66077-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66077-1-Ig (TELO2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TELO2 Monoclonal antibody (66077-1-Ig)

    IHC staining of human kidney using 66077-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66077-1-Ig (TELO2 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TELO2 Monoclonal antibody (66077-1-Ig)

    IHC staining of human liver cancer using 66077-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 66077-1-Ig(TELO2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TELO2 Monoclonal antibody (66077-1-Ig)

    IHC staining of human liver cancer using 66077-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 66077-1-Ig(TELO2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TELO2 Monoclonal antibody (66077-1-Ig)

    IF Staining of HepG2 using 66077-1-Ig

    Immunofluorescent analysis of () fixed HepG2 cells using 66077-1-Ig (TELO2 antibody) at dilution of 1:25.

Tested Applications

Positive WB detected inY79 cells, L02 cells, A431 cells, HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman kidney tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66077-1-Ig targets TELO2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TELO2 fusion protein Ag8834
Full Name TEL2, telomere maintenance 2, homolog (S. cerevisiae)
Calculated Molecular Weight 837 aa, 92 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberBC017188
Gene Symbol TELO2
Gene ID (NCBI) 9894
RRIDAB_11182835
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TELO2 gene encodes a telomere length regulation protein TEL2 homolog. TELO2 may be involved in telomere length regulation and can form TTT complex with TTI1 and TTI2. TTT complex is required to stabilize protein levels of PIKK family proteins and is involved in the cellular resistance to DNA damage stresses, like ionizing radiation (IR), and ultraviolet (UV). The activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals, can be promoted, stabilized and maintained by TELO2.

Protocols

Product Specific Protocols
WB protocol for TELO2 antibody 66077-1-IgDownload protocol
IHC protocol for TELO2 antibody 66077-1-IgDownload protocol
IF protocol for TELO2 antibody 66077-1-IgDownload protocol
IP protocol for TELO2 antibody 66077-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Inositol Pyrophosphates Mediate the DNA-PK/ATM-p53 Cell Death Pathway by Regulating CK2 Phosphorylation of Tti1/Tel2.

Authors - Feng Rao
View Article