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Anti-Human CD107b / LAMP2 (H4B4) Mouse IgG2a Recombinant Antibody

CD107b / LAMP2 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

H4B4

Cat no : 65566-1-MR

Synonyms

LAMP2, CD107b, H4B4, LAMP 2, CD107 antigen-like family member B, LAMP-2, LAMPB, LGP110, LGP-96, Lysosome-associated membrane glycoprotein 2, Lysosome-associated membrane protein 2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Jurkat cells using Anti-Human CD107b / LAMP2 (H4B4) Mouse Recombinant (65566-1-MR)

    FC experiment of Jurkat using 65566-1-MR

    1x10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human LAMP-2 (H4B4) Mouse Recombinant Antibody (65566-1-MR, Clone:H4B4) and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8)(red), or 0.5 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of Jurkat cells using Anti-Human CD107b / LAMP2 (H4B4) Mouse IgG2a Recom (65566-1-MR)

    FC experiment of Jurkat using 65566-1-MR

    1x10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human CD107b / LAMP2 (H4B4) Mouse IgG2a Recombinant Antibody (65566-1-MR, Clone: H4B4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.5 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC (Intra) detected inJurkat cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65566-1-MR targets CD107b / LAMP2 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human adherent peripheral blood cells
Full Name lysosomal-associated membrane protein 2
Calculated Molecular Weight 45 kDa
GenBank Accession NumberBC002965
Gene Symbol LAMP2
Gene ID (NCBI) 3920
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).

Protocols

Product Specific Protocols
FC protocol for CD107b / LAMP2 antibody 65566-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols