• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anti-Human CD16 (3G8), PBS Only

CD16 Monoclonal Antibody for Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G8

Cat no : 65090-1-PBS

Synonyms

Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A

Validation Data Gallery

Filter:
  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD16 (3G8) (65090-1-Ig)

    FC experiment of human PBMCs using 65090-1-Ig (same clone as 65090-1-PBS)

    1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD3 and 0.2 ug Anti-Human CD16 (65090-1-Ig, Clone:3G8) and APC-Goat anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65090-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD16 (3G8) (65090-1-Ig)

    FC experiment of human PBMCs using 65090-1-Ig (same clone as 65090-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD16 (65090-1-Ig, Clone:3G8) and APC-Goat anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65090-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50298-3

    Cytometric bead array standard curve of MP50298-3

    Cytometric bead array standard curve of MP50298-3, CD16 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 65090-1-PBS. Detection antibody: 68897-2-PBS. Standard:Eg31662. Range: 1.563-100 ng/mL.

  • Cytometric bead array standard curve of MP50298-4

    Cytometric bead array standard curve of MP50298-4

    Cytometric bead array standard curve of MP50298-4, CD16 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 65090-1-PBS. Detection antibody: 68897-3-PBS. Standard:Eg31662. Range: 0.781-100 ng/mL.

65090-1-Ig

Product Information

65090-1-PBS targets CD16 as part of a matched antibody pair:

MP50298-3: 65090-1-PBS capture and 68897-2-PBS detection (validated in Cytometric bead array)

MP50298-4: 65090-1-PBS capture and 68897-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Calculated Molecular Weight 254 aa, 29 kDa
GenBank Accession NumberBC017865
Gene Symbol CD16a
Gene ID (NCBI) 2214
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).