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PIAS1 Monoclonal antibody

PIAS1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

10A12E6

Cat no : 60498-1-Ig

Synonyms

EC:2.3.2.-, E3 SUMO-protein transferase PIAS1, E3 SUMO-protein ligase PIAS1, DEAD/H box-binding protein 1, DDXBP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PIAS1 Monoclonal antibody (60498-1-Ig)

    WB analysis using 60498-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60498-1-Ig (PIAS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using PIAS1 Monoclonal antibody (60498-1-Ig)

    WB analysis of THP-1 using 60498-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 60498-1-Ig (PIAS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMOLT-4 cells, THP-1 cells, Jurkat cells, IMR-32 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60498-1-Ig targets PIAS1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PIAS1 fusion protein Ag18132
Full Name protein inhibitor of activated STAT, 1
Calculated Molecular Weight 651 aa, 72 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC121797
Gene Symbol PIAS1
Gene ID (NCBI) 8554
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PIAS1 antibody 60498-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols