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EVI1 Monoclonal antibody

EVI1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A12A6

Cat no : 60413-1-Ig

Synonyms

MDS1 EVI1, EVI-1, EVI 1, EC:2.1.1.367, AML1 EVI 1

Validation Data Gallery

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  • Western Blot (WB) analysis of MKN-45 cells using EVI1 Monoclonal antibody (60413-1-Ig)

    WB analysis of MKN-45 using 60413-1-Ig

    MKN-45 cells were subjected to SDS PAGE followed by western blot with 60413-1-Ig (EVI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EVI1 Monoclonal antibody (60413-1-Ig)

    WB analysis using 60413-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60413-1-Ig (EVI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMKN-45 cells, Calu-3 cells, MDA-MB-231 cells, HeLa cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60413-1-Ig targets EVI1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EVI1 fusion protein Ag34143
Full Name ecotropic viral integration site 1
Calculated Molecular Weight 138 kDa
Observed Molecular Weight140-150 kDa
GenBank Accession NumberNM_001105077
Gene Symbol EVI1
Gene ID (NCBI) 2122
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EVI1 antibody 60413-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols