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WB analysis of human colon using 60351-1-Ig
Untreated and PNGase F-treated lysates of human colon tissue were subjected to SDS PAGE followed by western blot with 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).
WB analysis of pig colon using 60351-1-Ig
pig colon tissue were subjected to SDS PAGE followed by western blot with 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human colon using 60351-1-Ig
human colon tissue were subjected to SDS PAGE followed by western blot with 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 60351-1-Ig
Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 60351-1-Ig
Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 60351-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 60351-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 60351-1-Ig
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 60351-1-Ig
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 60351-1-Ig
Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 60351-1-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 60351-1-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human colon cancer using 60351-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Cadherin-17 antibody (60351-1-Ig, Clone: 3B10A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of human colon cancer using 60351-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Cadherin-17 antibody (60351-1-Ig, Clone: 3B10A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Cadherin-17 Monoclonal antibody

Cadherin-17 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 60351-1-Ig

Clone No.3B10A9

Publications(1)

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF-P, ELISA

Synonyms

CDH17, HPT1, HPT 1, CDH16, Cadherin 17

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	human colon tissue, pig colon tissue
Positive IHC detected in	human colon cancer tissue, human colon tissue, human pancreas cancer tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human colon cancer tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:20000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHC	See 1 publications below

Product Information

60351-1-Ig targets Cadherin-17 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity	human, pig
Cited Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	Cadherin-17 fusion protein Ag19488 Predict reactive species
Full Name	cadherin 17, LI cadherin (liver-intestine)
Calculated Molecular Weight	832 aa, 92 kDa
Observed Molecular Weight	120 kDa
GenBank Accession Number	BC113464
Gene Symbol	Cadherin-17
Gene ID (NCBI)	1015
RRID	AB_2881460
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q12864
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Cadherin-17 antibody 60351-1-Ig	Download protocol
IHC protocol for Cadherin-17 antibody 60351-1-Ig	Download protocol
IF protocol for Cadherin-17 antibody 60351-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Biochim Biophys Acta Gen Subj Gastric cancer patient-derived organoids model for the therapeutic drug screening Authors - Jiao Xu View Article