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Gastrin Monoclonal antibody

Gastrin Monoclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

7G9C3

Cat no : 60346-1-Ig

Synonyms

G14 Gastrin 6, G17 Gastrin 14, G34 Gastrin, G52 Big gastrin, G6, GAS, GAST, gastrin, Gastrin 17, Gastrin 34, Gastrin component I Gastrin 52, Gastrin component II, Gastrin component III

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human stomach tissue using Gastrin Monoclonal antibody (60346-1-Ig)

    IHC staining of human stomach using 60346-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 60346-1-Ig (Gastrin antibody) at dilution of 1:350 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using Gastrin Monoclonal antibody (60346-1-Ig)

    IHC staining of human stomach using 60346-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 60346-1-Ig (Gastrin antibody) at dilution of 1:350 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human stomach cancer tissue using Gastrin Monoclonal antibody (60346-1-Ig)

    IF Staining of human stomach cancer using 60346-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human stomach cancer tissue using Gastrin antibody (60346-1-Ig, Clone: 7G9C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman stomach cancer tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:100-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60346-1-Ig targets Gastrin in IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Gastrin fusion protein Ag12795
Full Name gastrin
Calculated Molecular Weight 101 aa, 11 kDa
GenBank Accession NumberBC069724
Gene Symbol Gastrin
Gene ID (NCBI) 2520
RRIDAB_2881455
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gastrin is a hormone produced primarily by G-cells in the stomach, where it functions to stimulate acid secretion by gastric parietal cells. Gastrin is a promiscuous hormone. Thus, the gastrin gene is expressed in common cancers such as bronchogenic, colorectal, ovarian and pancreatic carcinomas.

Protocols

Product Specific Protocols
IHC protocol for Gastrin antibody 60346-1-IgDownload protocol
IF protocol for Gastrin antibody 60346-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell

High-Resolution mRNA and Secretome Atlas of Human Enteroendocrine Cells.

Authors - Joep Beumer
View Article
humanIHC

Virchows Arch

Mixed adenoma-neuroendocrine tumor of the stomach: analysis of nine cases with literature review

Authors - Hirofumi Rokutan
View Article