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- KD/KO Validated
GOT1 Monoclonal antibody
GOT1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, chicken
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
CloneNo.
5A12E10
Cat no : 60317-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | LNCaP cells, rat brain tissue, rat heart tissue, mouse brain tissue, K-562 cells, chicken brain, chicken heart |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
60317-1-Ig targets GOT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, chicken samples.
| Tested Reactivity | human, mouse, rat, chicken |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GOT1 fusion protein Ag7012 |
| Full Name | glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 40-46 kDa |
| GenBank Accession Number | BC000498 |
| Gene Symbol | GOT1 |
| Gene ID (NCBI) | 2805 |
| RRID | AB_2881428 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GOT1 antibody 60317-1-Ig | Download protocol |
| IHC protocol for GOT1 antibody 60317-1-Ig | Download protocol |
| IF protocol for GOT1 antibody 60317-1-Ig | Download protocol |
| FC protocol for GOT1 antibody 60317-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Mol Med HIF-2α regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway in human pancreatic ductal adenocarcinoma. | ||
Nat Cell Biol De novo pyrimidine biosynthetic complexes support cancer cell proliferation and ferroptosis defence
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