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  • Featured Product
  • KD/KO Validated

IL-20RB Monoclonal antibody

IL-20RB Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A2A3

Cat no : 60221-1-Ig

Synonyms

DIRS1, FNDC6, IL 20 receptor subunit beta, IL 20R beta, IL 20R2, IL 20RB, IL20RB, IL-20RB, interleukin 20 receptor beta

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-20RB Monoclonal antibody (60221-1-Ig)

    WB analysis using 60221-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60221-1-Ig (IL-20RB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-10A cells using IL-20RB Monoclonal antibody (60221-1-Ig)

    WB analysis of MCF-10A using 60221-1-Ig

    MCF-10A cells were subjected to SDS PAGE followed by western blot with 60221-1-Ig (IL-20RB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA431 cells, MCF-10A cells, A375 cells, hTERT-RPE1 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60221-1-Ig targets IL-20RB in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen IL-20RB fusion protein Ag14368
Full Name interleukin 20 receptor beta
Calculated Molecular Weight 311 aa, 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC033292
Gene Symbol IL-20RB
Gene ID (NCBI) 53833
RRIDAB_11182930
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-20 receptor subunit beta (IL20RB) is a single-pass type I membrane protein of the type II cytokine receptor family. The interleukin-20-receptor I complex (IL-20-RI) is composed of two chains, IL20RA and IL20RB. IL-20-RI is a receptor for IL-19, IL-20 and IL-24. IL20RB can also form a heterodimer with IL22RA1. The IL22RA1/IL20RB dimer is a receptor for IL20 and IL24.

Protocols

Product Specific Protocols
WB protocol for IL-20RB antibody 60221-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

A systematic CRISPR screen reveals an IL-20/IL20RA-mediated immune crosstalk to prevent the ovarian cancer metastasis.

Authors - Jia Li
  • KD Validated
View Article