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ZAP70 Monoclonal antibody

ZAP70 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A11G9

Cat no : 60200-1-Ig

Synonyms

70 kDa zeta associated protein, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ZAP70 Monoclonal antibody (60200-1-Ig)

    WB analysis of Jurkat using 60200-1-Ig

    WB result of ZAP70 antibody (60200-1-Ig, 1:10000) with si-control and si-ZAP70 transfected Jurkat cells..

  • Western Blot (WB) analysis of Jurkat cells using ZAP70 Monoclonal antibody (60200-1-Ig)

    WB analysis of Jurkat using 60200-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig spleen tissue using ZAP70 Monoclonal antibody (60200-1-Ig)

    WB analysis of pig spleen using 60200-1-Ig

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig thymus tissue using ZAP70 Monoclonal antibody (60200-1-Ig)

    WB analysis of pig thymus using 60200-1-Ig

    pig thymus tissue were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ZAP70 Monoclonal antibody (60200-1-Ig)

    WB analysis of Jurkat using 60200-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ZAP70 Monoclonal antibody (60200-1-Ig)

    IHC staining of human lymphoma using 60200-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ZAP70 Monoclonal antibody (60200-1-Ig)

    IHC staining of human lymphoma using 60200-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ZAP70 Monoclonal antibody (60200-1-Ig)

    IHC staining of human tonsillitis using 60200-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ZAP70 Monoclonal antibody (60200-1-Ig)

    IHC staining of human tonsillitis using 60200-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using ZAP70 Monoclonal antibody (60200-1-Ig)

    IF Staining of human tonsillitis using 60200-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60200-1-Ig (ZAP70 antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, pig spleen tissue, pig thymus tissue
Positive IHC detected inhuman lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

60200-1-Ig targets ZAP70 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ZAP70 fusion protein Ag7943
Full Name zeta-chain (TCR) associated protein kinase 70kDa
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC039039
Gene Symbol ZAP70
Gene ID (NCBI) 7535
RRIDAB_10944567
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.

Protocols

Product Specific Protocols
WB protocol for ZAP70 antibody 60200-1-IgDownload protocol
IHC protocol for ZAP70 antibody 60200-1-IgDownload protocol
IF protocol for ZAP70 antibody 60200-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Cell volume controlled by LRRC8A-formed volume-regulated anion channels fine-tunes T cell activation and function

Authors - Yuman Wang
View Article
humanWB

Cell Immunol

Cbl-b inhibition promotes less differentiated phenotypes of T cells with enhanced cytokine production

Authors - Junfan Wang
View Article