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MSH2 Monoclonal antibody

MSH2 Monoclonal Antibody for IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E4A7

Cat no : 60161-2-Ig

Synonyms

MSH 2, 2E4A7

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  • Immunohistochemistry (IHC) staining of Ramos cells using MSH2 Monoclonal antibody (60161-2-Ig)

    IHC staining of Ramos using 60161-2-Ig

    Immunohistochemical analysis of paraffin-embedded Ramos cells slide using 60161-2-Ig (MSH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MSH2 Monoclonal antibody (60161-2-Ig)

    IHC staining of human tonsillitis using 60161-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60161-2-Ig (MSH2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inRamos cells, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60161-2-Ig targets MSH2 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MSH2 fusion protein Ag7835
Full Name mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
Calculated Molecular Weight 105 kDa
Observed Molecular Weight105 kDa
GenBank Accession NumberBC021566
Gene Symbol MSH2
Gene ID (NCBI) 4436
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A Magarose purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.

Protocols

Product Specific Protocols
IHC protocol for MSH2 antibody 60161-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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