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Gastrokine 1 Monoclonal antibody

Gastrokine 1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, pig, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3C10E3

Cat no : 60130-1-Ig

Synonyms

18 kDa antrum mucosa protein, AMP 18, AMP18, BRICD1, CA11, FOV, foveolin, gastrokine 1, GKN1, Protein CA11

Validation Data Gallery

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  • Western Blot (WB) analysis of human stomach tissue using Gastrokine 1 Monoclonal antibody (60130-1-Ig)

    WB analysis of human stomach using 60130-1-Ig

    human stomach tissue were subjected to SDS PAGE followed by western blot with 60130-1-Ig (GKN1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat stomach tissue using Gastrokine 1 Monoclonal antibody (60130-1-Ig)

    WB analysis of rat stomach using 60130-1-Ig

    rat stomach tissue were subjected to SDS PAGE followed by western blot with 60130-1-Ig (GKN1 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using Gastrokine 1 Monoclonal antibody (60130-1-Ig)

    IHC staining of human stomach using 60130-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 60130-1-Ig (GKN1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using Gastrokine 1 Monoclonal antibody (60130-1-Ig)

    IHC staining of human stomach using 60130-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 60130-1-Ig (GKN1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human stomach tissue using Gastrokine 1 Monoclonal antibody (60130-1-Ig)

    IF Staining of human stomach using 60130-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human stomach tissue using Gastrokine 1 antibody (60130-1-Ig, Clone: 3C10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human stomach tissue using Gastrokine 1 Monoclonal antibody (60130-1-Ig)

    IF Staining of human stomach using 60130-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human stomach tissue using Gastrokine 1 antibody (60130-1-Ig, Clone: 3C10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse stomach tissue using Gastrokine 1 Monoclonal antibody (60130-1-Ig)

    IF Staining of mouse stomach using 60130-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using Gastrokine 1 antibody (60130-1-Ig, Clone: 3C10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse stomach tissue using Gastrokine 1 Monoclonal antibody (60130-1-Ig)

    IF Staining of mouse stomach using 60130-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using Gastrokine 1 antibody (60130-1-Ig, Clone: 3C10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman stomach tissue, rat stomach tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman stomach tissue, mouse stomach tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60130-1-Ig targets Gastrokine 1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat, pig, mouse samples.

Tested Reactivity human, rat, pig, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Gastrokine 1 fusion protein Ag6091
Full Name gastrokine 1
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC059778
Gene Symbol Gastrokine 1
Gene ID (NCBI) 56287
RRIDAB_2111459
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gastrokine 1 (GKN1), a stomach-specific protein also known as 18 kDa antrum mucosa protein (AMP-18) or foveolin, belongs to the gastrokine family of gastric mucus cell-secreted proteins. The human GKN1 gene has been localized in a region of chromosome 2p13 of about 6 kb and contains 6 exons. GKN1 is expressed only in normal human stomach, but is absent from gastric adenocarcinomas, gastro-esophageal adenocarcinoma cell lines, and other normal and tumor gastro-intestinal tissues. GKN1may play an important role in normal gastric function and may be a gastric tumour suppressor.

Protocols

Product Specific Protocols
WB protocol for Gastrokine 1 antibody 60130-1-IgDownload protocol
IHC protocol for Gastrokine 1 antibody 60130-1-IgDownload protocol
IF protocol for Gastrokine 1 antibody 60130-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols