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CTAGE1 Monoclonal antibody

CTAGE1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3B4C2

Cat no : 60087-1-Ig

Synonyms

Cancer/testis antigen 21.2, CT21.1, CT21.2, CT21.2,CTAGE2, CTAGE, CTAGE 1, CTAGE 2, CTAGE1, CTAGE2, Protein cTAGE 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CTAGE1 Monoclonal antibody (60087-1-Ig)

    WB analysis of HeLa using 60087-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60087-1-Ig (CTAGE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CTAGE1 Monoclonal antibody (60087-1-Ig)

    IHC staining of mouse testis using 60087-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 60087-1-Ig (CTAGE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CTAGE1 Monoclonal antibody (60087-1-Ig)

    IHC staining of mouse testis using 60087-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 60087-1-Ig (CTAGE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CTAGE1 Monoclonal antibody (60087-1-Ig)

    IF Staining of MCF-7 using 60087-1-Ig

    Immunofluorescent analysis of MCF-7 cells, using CTAGE1 antibody 60087-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60087-1-Ig targets CTAGE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CTAGE1 fusion protein Ag3948
Full Name cutaneous T-cell lymphoma-associated antigen 1
Calculated Molecular Weight 754 aa, 89 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC031065
Gene Symbol CTAGE1
Gene ID (NCBI) 64693
RRIDAB_2086620
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CTAGE1 antibody 60087-1-IgDownload protocol
IHC protocol for CTAGE1 antibody 60087-1-IgDownload protocol
IF protocol for CTAGE1 antibody 60087-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols