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  • KD/KO Validated

STOML2 Monoclonal antibody

STOML2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1A2E9

Cat no : 60052-1-Ig

Synonyms

SLP 2, Paratarg-7, Paraprotein target 7, EPB72-like protein 2, EPB72 like protein 2



Tested Applications

Positive WB detected inpig brain tissue, HepG2 cells, MCF-7 cells, RAW 264.7 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman stomach cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60052-1-Ig targets STOML2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen STOML2 fusion protein Ag0363
Full Name stomatin (EPB72)-like 2
Calculated Molecular Weight 356 aa, 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC002442
Gene Symbol STOML2
Gene ID (NCBI) 30968
RRIDAB_2271235
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human stomatin (band 7.2b) is a 31-kDa erythrocyte membrane protein of unknown function but implicated in the control of ion channel permeability, mechanoreception, and lipid domain organization. Stomatin (EPB72)-like 2 (STOML2, synonyms: SLP-2, HSPC108) is a 38.5-kDa protein that is overall approximately 20% similar to human stomatin. STOML2 is also present in mature human erythrocytes , but lacks a characteristic NH(2)-terminal hydrophobic domain found in other stomatin homologues. STOML2 may link stomatin or other integral membrane proteins to the peripheral cytoskeleton and thereby play a role in regulating ion channel conductances or the organization of sphingolipid and cholesterol-rich lipid rafts.

Protocols

Product Specific Protocols
WB protocol for STOML2 antibody 60052-1-IgDownload protocol
IHC protocol for STOML2 antibody 60052-1-IgDownload protocol
IF protocol for STOML2 antibody 60052-1-IgDownload protocol
IP protocol for STOML2 antibody 60052-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Science

Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.

Authors - Shun Nagashima
  • KD Validated
humanWB

Mol Cell Proteomics

An informatics-assisted label-free approach for personalized tissue membrane proteomics: case study on colorectal cancer.

Authors - Han Chia-Li CL
human,mouseIF,IP

Int J Mol Sci

The Mitochondrial PHB2/OMA1/DELE1 Pathway Cooperates with Endoplasmic Reticulum Stress to Facilitate the Response to Chemotherapeutics in Ovarian Cancer.

Authors - Meiyu Cheng
humanIHC

Int J Cancer

Novel prognostic protein markers of resectable pancreatic cancer identified by coupled shotgun and targeted proteomics using formalin-fixed paraffin-embedded tissues.

Authors - Takadate Tatsuyuki T
humanWB

Cell Death Dis

Stomatin-like protein 2 regulates survivin expression in non-small cell lung cancer cells through β-catenin signaling pathway.

Authors - Cheng-Ta Yang
  • KD Validated
humanWB

J Cell Sci

Deficiency of PHB complex impairs respiratory supercomplex formation and activates mitochondrial flashes.

Authors - Chongshu Jian
  • KD Validated