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ESR2 Beta 3 Polyclonal antibody

ESR2 Beta 3 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 55441-1-AP

Synonyms

ER BETA, Erb, ESR BETA, ESR2, ESR2 Beta 3, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human cervix tissue using ESR2 Beta 3 Polyclonal antibody (55441-1-AP)

    IHC staining of human cervix using 55441-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervix tissue using ESR2 Beta 3 Polyclonal antibody (55441-1-AP)

    IHC staining of human cervix using 55441-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervix tissue using ESR2 Beta 3 Polyclonal antibody (55441-1-AP)

    IHC staining of human cervix using 55441-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervix tissue using ESR2 Beta 3 Polyclonal antibody (55441-1-AP)

    IHC staining of human cervix using 55441-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using ESR2 Beta 3 Polyclonal antibody (55441-1-AP)

    IHC staining of human endometrial cancer using 55441-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using ESR2 Beta 3 Polyclonal antibody (55441-1-AP)

    IHC staining of human endometrial cancer using 55441-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ESR2 Beta 3 Polyclonal antibody (55441-1-AP)

    IHC staining of human breast cancer using 55441-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ESR2 Beta 3 Polyclonal antibody (55441-1-AP)

    IHC staining of human breast cancer using 55441-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ESR2 Beta 3 Polyclonal antibody (55441-1-AP)

    IF Staining of HeLa using 55441-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ESR2 Beta 3 antibody (55441-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red) DAPI (blue).

Tested Applications

Positive IHC detected inhuman cervix tissue, human breast cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55441-1-AP targets ESR2 Beta 3 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name estrogen receptor 2 (ER beta)
Calculated Molecular Weight 58 kDa
GenBank Accession NumberNM_001437
Gene Symbol ESR2
Gene ID (NCBI) 2100
RRIDAB_2881334
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual

Protocols

Product Specific Protocols
IHC protocol for ESR2 Beta 3 antibody 55441-1-APDownload protocol
IF protocol for ESR2 Beta 3 antibody 55441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols