Tested Applications
Positive WB detected in | mouse spleen tissue, rat spleen tissue |
Positive IHC detected in | mouse spleen tissue, rat spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse spleen tissue, mouse colon tissue, rat spleen tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
31909-1-AP targets CD20 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.
Tested Reactivity | mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CD20 fusion protein Ag36918 Predict reactive species |
Full Name | membrane-spanning 4-domains, subfamily A, member 1 |
Calculated Molecular Weight | 32 kDa |
Observed Molecular Weight | 35 kDa |
GenBank Accession Number | NM_007641 |
Gene Symbol | Ms4a1 |
Gene ID (NCBI) | 12482 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity Purification |
UNIPROT ID | P19437 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as a calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CD20 antibody 31909-1-AP | Download protocol |
IHC protocol for CD20 antibody 31909-1-AP | Download protocol |
IF protocol for CD20 antibody 31909-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |