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IKBKAP Polyclonal antibody

IKBKAP Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31705-1-AP

Synonyms

IKK complex associated protein, IKAP, FLJ12497, ELP1, Elongator complex protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IKBKAP Polyclonal antibody (31705-1-AP)

    WB analysis using 31705-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31705-1-AP (IKBKAP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, U-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31705-1-AP targets IKBKAP in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IKBKAP fusion protein Ag35286
Full Name inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein
Calculated Molecular Weight150kDa，1332aa
Observed Molecular Weight140-150 kDa
GenBank Accession NumberNM_001318360.1
Gene Symbol IKBKAP
Gene ID (NCBI) 8518
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IKBKAP, also known as ELP1. It is expected to be located in the cytoplasm and nucleus, which is ubiquitinated in adrenal and ovary tissue. The protein encoded by this gene is a scaffold protein and a regulator for three different kinases involved in proinflammatory signaling. The encoded protein can bind NF-kappa-B-inducing kinase and I-kappa-B kinases through separate domains and assemble them into an active kinase complex. Mutations in this gene have been associated with familial dysautonomia. And it exists in homodimer. The molecular weight of IKBKAP is 150 kDa.

Protocols

Product Specific Protocols
WB protocol for IKBKAP antibody 31705-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols